Dị ka ihe oriri na-adịghị ahụkebe na ero ọgwụ, black truffle (Tuber melanosporum) ọ bụghị nanị na ọ nwere ekpomeekpo pụrụ iche, kamakwa ọ bara ụba na ihe ndị na-emepụta ihe na-arụ ọrụ nke ọma dị ka polysaccharides na L-ergothioneineineineineineineine. Nnyocha nke oge a na-egosi na ihe ndị a nwere ọrụ dị iche iche physiological dị ka antioxidant, mgbochi mkpali, mgbochi ịka nká, na usoro nchịkwa, nke na-eme ka truffles ojii na-egosi nnukwu ngwa ngwa n'ọhịa nke ịchọ mma na nri na-arụ ọrụ.

Agbanyeghị, mmepe akụrụngwa ya echewo ihe mgbochi abụọ dị mkpa ihu ogologo oge:

1. Ngwa ndị na-edoghị anya: Ọ bụ ezie na a na-akpọ truffles ojii dị ka "obere", ihe ndị dị n'ime ha bụ isi ihe na-arụ ọrụ (dị ka polysaccharides na L-ergothioneineineineineineineine) adịghị edozi, na-akpata enweghị nkwado data ziri ezi maka nkwupụta nrụpụta ngwaahịa na ihe isi ike na ịnọgide na-adị mma mgbe niile;
2. Mmalite dabere na ahụmịhe: Nhọrọ nke akụrụngwa na-adaberekarị na ahụmịhe ọdịnala ma ọ bụ aha sitere na mmalite. Enweghi ihe omuma ihe omuma sayensi dika ihe ndabere maka nhoputa, na-ebute enweghi ike imezu ihe omuma nke oma maka ihe di iche iche, na uru nke ihe ndi ozo enweghi ike ibuli elu.

Iji dozie isi ihe mgbu ndị a dị n'elu, Landivine Research Institute jiri ụzọ ọhụrụ tinye mmiri chromatography-tandem mass spectrometry (LC-MS/MS) usoro nha nha anya na nyocha nhazi nke akụrụngwa ojii. Ebipụtala akwụkwọ ndị metụtara ya na akwụkwọ akụkọ agụmakwụkwọ.

Site n'ịmepụta usoro nchọpụta dị oke mmetụta na nke nwere ike imepụtaghachi, ọmụmụ a nwetara na nke mbụ "ihe osise" ziri ezi nke L-ergothioneineineineineineineineineine na polysaccharide ọdịnaya na truffles ojii sitere na mmalite dị iche iche dị ka Liaoning na Yunnan, na-enye ihe ndabere sayensị siri ike maka mmepe na ntinye nke akụrụngwa.

Ọganihu teknụzụ

Iji nweta nkọwa ziri ezi nke isi ihe dị na truffles ojii, ọmụmụ ihe a jiri mmiri chromatography-tandem mass spectrometry (LC-MS/MS) na usoro phenol-sulfuric acid iji nyochaa usoro L-ergothioneineineineineineine na polysaccharide ọdịnaya nke black truffles sitere na Anshan, Goming Yunnaning, na Kunnnan.

N'ịchọpụta ọdịnaya nke L-ergothioneineineineineineineineine na black truffles, usoro LC-MS/MS ejiri mee ihe n'ọmụmụ ihe a na-egosi uru ndị a bụ isi:

1. Mmetụta dị elu na oke ahịrị obosara: Usoro a nwere mmekọrịta dị mma n'ahịrị n'ime oke ntinye nke 0.01 ~ 1.0μg / mL, na njedebe dị ala nke quantification dị ka 0.01μg / ml. Nke a na-egosi na usoro a nwere ike itinye aka na nkwụsi ike;
2. Ezigbo ezi na izi ezi: Nsonaazụ nyocha nke usoro na-egosi na, ewezuga mkpokọta L-ergothioneineineineineineineineineine nke mkpokọta nke 10ng/mL, ngbanwe nke ụkpụrụ niile anwalere bụ <3%, na ọnụego mgbake nke nlele ahụ dị n'etiti 92.80% na 104.20%. Nke a na-egosi na usoro ahụ nwere ezigbo nkwughachi, nkenke dị elu, izi ezi na ntụkwasị obi, ma na-ezute ihe nyocha nke ọma.

Nchọpụta isi

Ọmụmụ ihe a mere nyocha nhazi nke truffles ojii sitere na Anshan, Liaoning, Kunming, Yunnan, na Gongshan, Yunnan, wee chọpụta na e nwere nnukwu ọdịiche mpaghara na ọdịnaya nke isi ihe ha na-arụ ọrụ.

Ntụle nke ọdịnaya polysaccharide na truffles ojii

Polysaccharide bụ otu n'ime ihe dị mkpa na-arụ ọrụ nke truffle ojii ma nwee ezigbo ọrụ antioxidant na ọrụ hypoglycemic. Ọmụmụ ihe ahụ jiri usoro phenol-sulfuric acid mee mkpebi.

• Ọdịnaya polysaccharide nke mpụta mmiri: Mgbe mmịpụta mmiri kpọmkwem, ọdịnaya polysaccharide nke truffles ojii sitere na Anshan, Liaoning Province bụ nke kachasị elu, Kunming na-esote, Yunnan na n'ikpeazụ Gongshan, Yunnan.

• Ọdịnaya polysaccharide nke mmanya na-ebuli: Mgbe mmiri ozuzo ethanol na ime ka ọ dị ọcha, ọdịnaya polysaccharide nke truffles ojii na mpaghara dị iche iche mụbara, na ọkwa ọdịnaya gbanwere: ọdịnaya polysaccharide nke black truffles na Gongshan, Yunnan bụ nke kachasị elu, Kunming, Yunnan na n'ikpeazụ Anshan, Liaoning.

Ntụle ọdịnaya L-ergothioneineineineinineine

L-ergothioneineineineineineineine, dị ka ihe nchekwa, nke na-adịghị egbu egbu, nke na-arụ ọrụ thiohistidine multi-functional, nwere ọtụtụ mmetụta dị ka mgbochi mkpali, ịgbatị ogologo ndụ ndụ cell, na ịkwalite nhazi nke mkpụrụ ndụ akwara. Ọmụmụ ihe a jiri usoro chromatography-tandem mass spectrometry (LC-MS/MS) mee mkpebi.

• Nsonaazụ nha ọdịnaya na-egosi na truffles ojii sitere na Kunming, Yunnan, nwere ọdịnaya L-ergothioneineineineineineineineine, nke dị elu karịa ọdịnaya L-ergothioneineineineineineineine na truffles ojii sitere na Gongshan, Yunnan na Anshan, Liaoning. Nke a na-egosi n'ụzọ doro anya na truffles ojii sitere na Kunming, Yunnan, nwere ikike dị ukwuu na ngwa antioxidant na mgbochi ịka nká.

Atụmanya ngwa

Nsonaazụ data nke ọmụmụ a na-enye nkwado nkwado siri ike na nkwado data maka mmepe ziri ezi na ntinye nke black truffle n'ọhịa nke ịchọ mma, ọgwụ na nri.

1. Nyocha ntụzịaka nke akụrụngwa: Iji zụlite ngwaahịa na-elekwasị anya na mmiri na-arụzi ọrụ, ihe ndị sitere na Yunnan Gongshan nwere nnukwu mmanya na-aba n'anya polysaccharide nwere ike ịhọrọ; Ọ bụrụ na isi mkpesa bụ antioxidant, akụrụngwa sitere na Yunnan Kunming nwere ọdịnaya L-ergothioneineineineineineineineine kachasị elu kwesịrị ka ahọrọ.
2. Nhazi njikwa mma: Usoro nchọpụta LS-MS/MS nke e guzobere n'ọmụmụ ihe a bụ nke ziri ezi ma bụrụ nke a pụrụ ịdabere na ya, enwere ike iji ya mee ihe dị ka ụzọ ọrụ nkà na ụzụ isi maka njikwa mma na nhazi nke ngwaahịa truffle ojii na ihe ndị na-emepụta ya, na-eme ka nkwụsi ike na njikwa nke nrụpụta ngwaahịa.
3. Nkwanye ike ngwaahịa: Nnyocha ekwenyela na truffles ojii nke China bara ụba na ihe ndị na-arụsi ọrụ ike dị elu, na-enye nkwado sayensị siri ike maka ngwa ọhụrụ ya na ngwaahịa nlekọta anụ ahụ dị elu, nri na-arụ ọrụ, na uwe ọgwụ.

Nchịkọta nyocha

Ọmụmụ ihe a na-enyocha usoro nke polysaccharide na L-ergothioneineineineineineineine nke dị na truffles ojii sitere na mmalite dị iche iche, ma kpughee na truffles ojii sitere na Yunnan rụrụ nke ọma n'ihe gbasara L-ergothioneineineineineineineine na polysaccharides na-ebuli mmanya. N'ime ha, mmalite Kunming nwere ọdịnaya L-ergothioneineineineineineineineine kachasị elu, na mmalite Gongshan nwere ọdịnaya polysaccharide na-ebuli mmanya na-aba n'anya.

E jiri ya tụnyere ngwa ọdịnala nwere otu ihe na-arụ ọrụ, mwepu truffle ojii nwere ma nrụzi na ikike antioxidant nke polysaccharides na L-ergothioneineineineineineineine, na-enweta mmekọrịta okike nke ọtụtụ ọrụ physiological. Ọmụmụ ihe a na-enye ndabere a pụrụ ịdabere na ya maka nhọrọ ziri ezi na ịdị irè nke black truffle na ngwaahịa nlekọta anụ ahụ dị elu na nri na-arụ ọrụ, ma na-atọkwa ntọala sayensị maka mmepe dị omimi na njikwa mma nke ihe ndị sitere n'okike.