Ma ke ʻano he mea ʻai ʻole a me ka lāʻau lapaʻau, ʻaʻole wale kahi ʻono kūʻokoʻa o ka truffle ʻeleʻele (Tuber melanosporum), akā he waiwai pū nō hoʻi i nā mea hana kūlohelohe kiʻekiʻe e like me polysaccharides a me L-ergothioneineineineineineineine. Hōʻike ka noiʻi hou i kēia mau mea i loaʻa i nā hana physiological like ʻole e like me ka antioxidant, anti-inflammatory, anti-aging, a me ka hoʻoponopono immune, kahi e hōʻike ai nā truffle ʻeleʻele i ka hiki ke hoʻohana nui ʻia i nā ʻano meaʻai a me nā meaʻai.

Eia nō naʻe, ua kū lōʻihi kona hoʻomohala ʻana i nā mea waiwai ʻelua:

1. ʻO nā mea i maopopo ʻole: ʻOiai ua kapa ʻia ʻo truffles ʻeleʻele he "rare", ʻaʻole maopopo nā kikoʻī kikoʻī o kā lākou mau mea hana nui (e like me polysaccharides a me L-ergothioneineineineineineineine), e hopena i ka nele o ke kākoʻo ʻikepili pololei no nā koi pono huahana a paʻakikī i ka mālama ʻana i ka maikaʻi mau;
2. Ke kumu ma muli o ka ʻike: ʻO ke koho ʻana i nā mea maka e hilinaʻi nui ʻia i ka ʻike kuʻuna a i ʻole ka inoa o ke kumu. Loaʻa ka nele o ka ʻikepili ʻepekema i kumu no ke koho ʻana, e hopena i ka hiki ʻole ke hoʻokō i ke koho pololei o nā mea no nā hopena kikoʻī, a ʻaʻole hiki ke hoʻonui ʻia ka waiwai o nā mea maka.

I mea e hoʻoponopono ai i nā ʻeha ʻeha ma luna, ua hoʻohana hou ʻo Landivine Research Institute i ka wai chromatography-tandem mass spectrometry (LC-MS/MS) i ke ʻano helu kikoʻī i ka loiloi ʻōnaehana o nā mea maka truffle ʻeleʻele. Ua paʻi kūhelu ʻia nā pepa pili i loko o nā puke pai palapala.

Ma ka hoʻokumu ʻana i kahi ala ʻike koʻikoʻi a hiki ke hana hou ʻia, ua loaʻa i kēia haʻawina no ka manawa mua i kahi "kiʻi" pololei o ka L-ergothioneineineineineineineine a me ka polysaccharide ma loko o nā truffle ʻeleʻele mai nā kumu like ʻole e like me Liaoning a me Yunnan, e hāʻawi ana i kahi kumu ʻepekema paʻa no ka hoʻomohala pololei a me ka hoʻohana ʻana i nā mea maka.

ʻO ka holomua ʻenehana

I mea e hoʻokō ai i ka helu kūpono o nā mea nui o nā truffles ʻeleʻele, ua hoʻohana kēia haʻawina i ka wai chromatography-tandem mass spectrometry (LC-MS/MS) a me ke ʻano phenol-sulfuric acid e hoʻopili pono ai i ka L-ergothioneineineineineineineine a me nā polysaccharide ma loko o nā ʻeleʻele truffles mai Anshan, Liaoning, Yunnanng, Yunnanng, Yunnanng, Kunming.

I ka ʻike ʻana i ka maʻiʻo o L-ergothioneineineineineineineine i loko o nā truffles ʻeleʻele, ʻo ke ʻano LC-MS/MS i hoʻohana ʻia ma kēia haʻawina e hōʻike ana i nā pono kumu:

1. ʻO ka ʻike kiʻekiʻe a me ka laulā laina ākea: He pilina laina maikaʻi kēia ʻano i loko o ka pae hoʻopaʻa ʻana o 0.01 ~ 1.0μg / mL, a ʻo ka palena haʻahaʻa o ka quantification e like me 0.01μg / mL. Hōʻike kēia i ka hoʻohana ikaika ʻana a me ke kūpaʻa o kēia ʻano;
2. Ka pololei a me ka pololei: Hōʻike nā hualoaʻa hoʻokolohua methodological, koe wale no ka L-ergothioneineineineineineineineine nuipa o 10ng/mL, ka mea pili maʻamau deviation o nā mea a pau i ho'āʻo hoʻopaʻa 'ia he <3%, a me ka hoʻihoʻi 'ana o ka hāpana ma waena o 92.80% a me 104.20%. Hōʻike kēia i ke ʻano o ka hana hou ʻana, ka pololei kiʻekiʻe, ka pololei a me ka hilinaʻi, a hoʻokō piha i nā koi loiloi.

Nā ʻike kumu

Ua alakaʻi kēia haʻawina i ka nānā ʻana o nā truffle ʻeleʻele mai Anshan, Liaoning, Kunming, Yunnan, a me Gongshan, Yunnan, a ua ʻike ʻia he mau ʻokoʻa koʻikoʻi o ka ʻāina i ka ʻike o kā lākou mea hana.

Ka hoʻohālikelike o ka polysaccharide ma loko o nā truffle ʻeleʻele

ʻO ka Polysaccharide kekahi o nā mea hana nui o ka truffle ʻeleʻele a he hana antioxidant maikaʻi a me ka hana hypoglycemic. Ua hoʻohana ka haʻawina i ke ʻano phenol-sulfuric acid no ka hoʻoholo ʻana.

• ʻO ka maʻi polysaccharide o ka wai wai: Ma hope o ka unuhi pololei ʻana i ka wai, ʻo ka nui o ka polysaccharide o nā ʻeleʻele truffles mai Anshan, Liaoning Province ka mea kiʻekiʻe loa, ukali ʻia e Kunming, Yunnan, a hope loa ʻo Gongshan, Yunnan.

• ʻO ka maʻi polysaccharide o ka waiʻona e hoʻoheheʻe: Ma hope o ka ua a me ka hoʻomaʻemaʻe ʻana i ka ethanol, ua hoʻonui ʻia ka ʻike polysaccharide o nā truffles ʻeleʻele ma nā wahi like ʻole, a ua hoʻololi ʻia ka helu helu: ʻo ka polysaccharide maʻiʻo o nā truffle ʻeleʻele ma Gongshan, ʻo Yunnan ka mea kiʻekiʻe loa, ukali ʻia e Kunming, Yunnan, a hope loa ʻo Anshan, Liaoning.

L-ergothioneineineineineineineine hoʻohālikelike maʻiʻo

ʻO L-ergothioneineineineineineineineine, he mea palekana, non-toxic, multi-functional thiohistidine derivative, he nui nā hopena e like me ka anti-inflammatory, hoʻolōʻihi i ka pōʻai ola cell, a me ka hoʻoikaika ʻana i ke kūkulu ʻana i nā pūnaewele nerve. Ua hoʻohana kēia haʻawina i ke ʻano chromatography-tandem mass spectrometry (LC-MS/MS) no ka hoʻoholo ʻana.

• Hōʻike nā hualoaʻa ana i ka ʻeleʻele truffles mai Kunming, Yunnan, i ka L-ergothioneineineineineineineine maʻiʻo kiʻekiʻe loa, ʻoi aku ka kiʻekiʻe ma mua o ka L-ergothioneineineineineineineine ma loko o nā truffle ʻeleʻele mai Gongshan, Yunnan a me Anshan, Liaoning. Hōʻike maopopo kēia i nā ʻeleʻele truffles mai Kunming, Yunnan, ʻoi aku ka nui o ka mana i nā noi antioxidant a me nā anti-aging.

Nā manaʻo noi

Hāʻawi nā hualoaʻa ʻikepili o kēia noiʻi i ke kākoʻo theoretical paʻa a me ke kākoʻo ʻikepili no ka hoʻomohala pololei a me ka hoʻohana ʻana i ka truffle ʻeleʻele ma nā ʻano mea hoʻonaninani, lāʻau lapaʻau a me nā meaʻai.

1. ʻO ka nānā ʻana o nā mea maka: No ka hoʻomohala ʻana i nā huahana e nānā ana i ka moisturizing a me ka hoʻoponopono ʻana i nā hana, hiki ke koho ʻia nā mea maka mai Yunnan Gongshan me ka nui o ka waiʻona-precipitated polysaccharide content; inā he antioxidant ka hoʻopiʻi koʻikoʻi, pono e makemake ʻia nā mea maka mai Yunnan Kunming me ka ʻike L-ergothioneineineineineineine kiʻekiʻe loa.
2. Hoʻoponopono hoʻoponopono maikaʻi: ʻO ke ala ʻike LS-MS / MS i hoʻokumu ʻia i loko o kēia haʻawina he pololei a hilinaʻi, a hiki ke hoʻohana ʻia ma ke ʻano he kumu ʻenehana koʻikoʻi no ka hoʻomalu maikaʻi ʻana a me ka hoʻohālikelike ʻana i nā mea waiwai truffle ʻeleʻele a me kāna huahana derivative, e hōʻoiaʻiʻo ana i ka kūpaʻa a me ka controllability o ka pono huahana.
3. Ka hoʻoikaika ʻana o ka huahana: Ua hōʻoia ka noiʻi ʻana he waiwai nui nā truffles ʻeleʻele maoli o Kina i nā mea hana waiwai nui, e hāʻawi ana i ka hōʻoia ʻepekema ikaika no kāna mau noi hou i nā huahana mālama ʻili kiʻekiʻe, nā meaʻai hana, a me nā lole lapaʻau.

Hōʻuluʻulu noiʻi

Keia haʻawina systematically kālailai i ka polysaccharide a me L-ergothioneineineineineineine maʻiʻo i loko o ka eleele truffles mai okoa kumu, a hoike mai i ka eleele truffles mai Yunnan hana maikai ma ka hua'ōlelo o L-ergothioneineineineineineineineine a me ka waiʻona-precipitated polysaccharides. Ma waena o lākou, ʻo ka kumu Kunming ka L-ergothioneineineineineineineine maʻiʻo kiʻekiʻe loa, a ʻo ka Gongshan kumu i loaʻa i ka polysaccharide waiʻona nui loa.

Ke hoʻohālikelike ʻia me nā mea maka kahiko me kahi mea hana hoʻokahi, loaʻa ka ʻeleʻele truffle extract i ka hoʻoponopono a me nā mana antioxidant o polysaccharides a me L-ergothioneineineineineineineine, e loaʻa ana i kahi synergy kūlohelohe o nā hana physiological he nui. Hāʻawi kēia haʻawina i kumu hilinaʻi no ke koho pololei a me ka pono o ka truffle ʻeleʻele i nā huahana mālama ʻili kiʻekiʻe a me nā meaʻai hana, a kau pū kekahi i kahi kumu ʻepekema no ka hoʻomohala hohonu a me ka hoʻokele maikaʻi ʻana o nā mea kūlohelohe.