Raws li ib qho tsis tshua muaj txiaj ntsig thiab tshuaj fungus, dub truffle (Tuber melanosporum) tsis tsuas yog muaj qhov tshwj xeeb tsw, tab sis kuj yog nplua nuj nyob rau hauv cov khoom muaj txiaj ntsig zoo xws li polysaccharides thiab L-ergothioneineineineineineineine. Cov kev tshawb fawb niaj hnub no qhia tau hais tias cov khoom xyaw no muaj ntau yam kev ua haujlwm ntawm lub cev xws li tshuaj tua kab mob antioxidant, tiv thaiv kab mob, tiv thaiv kev laus, thiab kev tiv thaiv kab mob, uas ua rau cov truffles dub qhia txog kev siv tau zoo hauv kev lag luam tshuaj pleev ib ce thiab khoom noj khoom haus.

Txawm li cas los xij, nws txoj kev txhim kho cov khoom siv raw tau ntev ntsib ob lub ntsiab lus tseem ceeb:

1. Cov khoom xyaw tsis meej: Txawm hais tias cov truffles dub dubbed "tsis tshua muaj", cov ntsiab lus tshwj xeeb ntawm lawv cov khoom xyaw tseem ceeb (xws li polysaccharides thiab L-ergothioneineineineineineineine) tsis meej, ua rau tsis muaj cov ntaub ntawv raug txhawb rau cov khoom siv tau zoo thiab nyuaj rau kev tswj xyuas qhov zoo;
2. Keeb kwm raws li kev paub: Kev xaiv cov khoom siv raw feem ntau yog nyob ntawm kev ua ib txwm muaj lossis lub koob npe nrov ntawm lub hauv paus chiv keeb. Muaj qhov tsis muaj cov ntaub ntawv tshawb fawb los ua lub hauv paus rau kev xaiv, ua rau tsis muaj peev xwm ua tiav qhov kev xaiv ntawm cov khoom siv rau cov teebmeem tshwj xeeb, thiab tus nqi ntawm cov khoom siv raw tsis tuaj yeem ua kom tiav.

Txhawm rau daws cov teeb meem saum toj no, Landivine Research Institute innovatively siv cov kua chromatography-tandem huab hwm coj spectrometry (LC-MS / MS) meej quantification txoj kev rau lub systematic ntsuam xyuas ntawm dub truffle raw cov ntaub ntawv. Cov ntaub ntawv cuam tshuam tau raug luam tawm hauv cov ntawv xov xwm kev kawm.

Los ntawm tsim txoj kev tshawb nrhiav tau zoo heev thiab muaj txiaj ntsig zoo, txoj kev tshawb fawb no tau ua tiav thawj zaug qhov tseeb "portrait" ntawm L-ergothioneineineineineineineineine thiab polysaccharide cov ntsiab lus hauv cov truffles dub los ntawm ntau lub hauv paus chiv keeb xws li Liaoning thiab Yunnan, muab lub hauv paus kev tshawb fawb zoo rau kev txhim kho thiab siv cov ntaub ntawv raw.

Kev paub txog technology

Txhawm rau kom ua tiav qhov tseeb ntawm cov khoom tseem ceeb ntawm cov truffles dub, txoj kev tshawb no tau siv cov kua chromatography-tandem huab hwm coj spectrometry (LC-MS/MS) thiab phenol-sulfuric acid txoj kev los txheeb xyuas L-ergothioneineineineineineineineine thiab polysaccharide cov ntsiab lus ntawm dub truffles los ntawm Gynnan, Anshan, thiab Anshanming. Yunnan.

Hauv kev kuaj xyuas cov ntsiab lus ntawm L-ergothioneineineineineineineineine hauv cov truffles dub, LC-MS / MS txoj kev siv hauv txoj kev tshawb no qhia txog cov txiaj ntsig hauv qab no:

1. High rhiab heev thiab dav linear ntau yam: Txoj kev no muaj kev sib raug zoo linear nyob rau hauv qhov concentration ntau ntawm 0.01 ~ 1.0μg / mL, thiab qis qis ntawm kev txiav txim siab yog tsawg li 0.01μg / mL. Qhov no qhia tau hais tias txoj kev no muaj zog applicability thiab stability;
2. Zoo heev precision thiab raug: Cov txiaj ntsig kev soj ntsuam tau pom tias, tshwj tsis yog rau L-ergothioneineineineineineineine cov concentration ntawm 10ng / mL, qhov sib txawv tus qauv sib txawv ntawm txhua qhov kev ntsuam xyuas yog <3%, thiab tus nqi rov qab ntawm tus qauv yog nyob nruab nrab ntawm 92.80% thiab 104.20%. Qhov no ua pov thawj tias txoj kev muaj qhov rov ua tau zoo, siab precision, raug thiab kev ntseeg tau, thiab ua tau raws li qhov xav tau ntawm kev tshuaj xyuas.

Cov kev tshawb pom tseem ceeb

Txoj kev tshawb no tau ua qhov kev soj ntsuam ntawm cov truffles dub los ntawm Anshan, Liaoning, Kunming, Yunnan, thiab Gongshan, Yunnan, thiab pom tias muaj qhov sib txawv hauv cheeb tsam tseem ceeb hauv cov ntsiab lus ntawm lawv cov khoom xyaw tseem ceeb.

Kev sib piv ntawm cov ntsiab lus polysaccharide hauv cov truffles dub

Polysaccharide yog ib qho ntawm cov khoom xyaw tseem ceeb ntawm cov truffle dub thiab muaj kev ua haujlwm zoo antioxidant thiab kev ua haujlwm hypoglycemic. Txoj kev tshawb no siv phenol-sulfuric acid txoj kev los txiav txim.

• Cov ntsiab lus ntawm polysaccharide dej extract: Tom qab cov dej ncaj qha rho tawm, cov ntsiab lus polysaccharide ntawm dub truffles los ntawm Anshan, Liaoning Province yog siab tshaj, ua raws li Kunming, Yunnan, thiab thaum kawg Gongshan, Yunnan.

• Cov ntsiab lus ntawm polysaccharide ntawm cawv precipitate: Tom qab ethanol los nag thiab purification, polysaccharide cov ntsiab lus ntawm dub truffles nyob rau hauv ntau lub cheeb tsam tau nce, thiab cov ntsiab lus qeb duas hloov: cov polysaccharide cov ntsiab lus ntawm dub truffles nyob rau hauv Gongshan, Yunnan yog siab tshaj, ua raws li Kunming, Yunnan, thiab thaum kawg Anshan, Liaoning.

L-ergothioneineineineineineineineine cov ntsiab lus sib piv

L-ergothioneineineineineineineine, raws li kev nyab xeeb, tsis muaj tshuaj lom, muaj ntau yam kev ua haujlwm thiohistidine derivative, muaj ntau yam teebmeem xws li tshuaj tiv thaiv, ua kom lub cev muaj sia nyob ntev, thiab txhawb kev tsim cov paj hlwb. Txoj kev tshawb no siv cov kua chromatography-tandem mass spectrometry (LC-MS/MS) txoj kev txiav txim.

• Cov ntsiab lus ntsuas tau pom tias cov truffles dub los ntawm Kunming, Yunnan, muaj cov ntsiab lus L-ergothioneineineineineineineineine siab tshaj plaws, uas yog siab tshaj L-ergothioneineineineineineineine cov ntsiab lus hauv cov truffles dub los ntawm Gongshan, Yunnan thiab Anshan, Liaoning. Qhov no qhia meej tias cov truffles dub los ntawm Kunming, Yunnan, muaj peev xwm ntau dua hauv kev siv tshuaj tua kab mob antioxidant thiab tiv thaiv kev laus.

Daim ntawv thov kev cia siab

Cov ntaub ntawv tau txais txiaj ntsig ntawm txoj kev tshawb no muab kev txhawb nqa theoretical thiab cov ntaub ntawv txhawb nqa rau kev txhim kho thiab kev siv cov truffle dub hauv kev lag luam tshuaj pleev ib ce, tshuaj thiab zaub mov.

1. Directional soj ntsuam ntawm raw cov ntaub ntawv: Txhawm rau tsim cov khoom lag luam tsom mus rau cov dej noo thiab kho cov haujlwm, cov khoom siv raw los ntawm Yunnan Gongshan nrog cov dej cawv-precipitated polysaccharide cov ntsiab lus yuav nyiam; Yog hais tias qhov tseem ceeb thov yog antioxidant, raw cov ntaub ntawv los ntawm Yunnan Kunming nrog siab tshaj L-ergothioneineineineineineineine cov ntsiab lus yuav tsum nyiam.
2. Quality tswj standardization: Txoj kev tshawb nrhiav LS-MS / MS tau tsim nyob rau hauv txoj kev tshawb no yog qhov tseeb thiab txhim khu kev qha, thiab tuaj yeem siv los ua cov txheej txheem tseem ceeb rau kev tswj kom zoo thiab kev ua qauv ntawm cov khoom siv dub truffle thiab nws cov khoom derivative, ua kom muaj kev ruaj ntseg thiab kev tswj hwm ntawm cov khoom siv tau zoo.
3. Product Efficiency empowerment: Kev tshawb fawb tau lees paub tias Tuam Tshoj haiv neeg dub truffles yog nplua nuj nyob rau hauv cov khoom xyaw uas muaj txiaj ntsig zoo, muab kev pom zoo rau kev tshawb fawb zoo rau nws cov kev siv tshiab hauv cov khoom siv kho mob siab, khoom noj khoom haus, thiab hnav khaub ncaws kho mob.

Cov ntsiab lus tshawb fawb

Txoj kev tshawb no tau txheeb xyuas cov polysaccharide thiab L-ergothioneineineineineineineineineine cov ntsiab lus hauv cov truffles dub los ntawm ntau lub hauv paus, thiab qhia tias cov truffles dub los ntawm Yunnan ua tau zoo ntawm L-ergothioneineineineineineineineine thiab cawv-precipitated polysaccharides. Ntawm lawv, Kunming keeb kwm muaj cov ntsiab lus L-ergothioneineineineineineineine ntau tshaj plaws, thiab Gongshan keeb kwm muaj cov ntsiab lus tseem ceeb tshaj plaws ntawm cov dej cawv-precipitated polysaccharide.

Piv nrog rau cov khoom siv raw nrog ib qho khoom muaj zog, dub truffle extract muaj ob qho tib si kho thiab antioxidant peev xwm ntawm polysaccharides thiab L-ergothioneineineineineineineineine, ua tiav ib qho kev sib koom ua ke ntawm ntau lub cev muaj zog. Txoj kev tshawb no muab lub hauv paus txhim khu kev qha rau cov kev xaiv meej thiab kev ua tau zoo ntawm cov truffle dub hauv cov khoom siv tawv nqaij zoo thiab cov khoom noj ua haujlwm, thiab tseem tsim lub hauv paus kev tshawb fawb rau kev txhim kho qhov tob thiab kev tswj xyuas zoo ntawm cov khoom siv raw.